商品介绍

Product Introduction

Bioactivity

名称	Cyanine5.5 maleimide chloride
描述	Cyanine5.5 maleimide chloride is a CY dye compound characterized by two nitrogen atoms connected through an odd number of methylene groups, exhibiting long excitation and emission wavelengths, high molar extinction coefficients, strong water solubility, and simple synthetic accessibility. Cyanine5.5 maleimide chloride is widely employed for the fluorescent labeling of proteins, antibodies, and small molecules, and the labeling process can be achieved through direct conjugation reactions that serve as a valuable reference model in bioimaging and analytical chemistry research.
体外活性	Cyanine5.5 maleimide chloride 是一种由 Cyanine5.5 荧光团和马来酰亚胺(maleimide）反应基团组成的共轭染料。Cyanine 5.5 是一种发近红外荧光的花青素荧光染料,广泛应用于小动物活体体内成像。它可用于标记蛋白质、抗体、多肽和纳米粒子等。马来酰亚胺(maleimide）是生物标记反应中常用的基团,能够与巯基(-SH）通过亲和加成反应生成稳定的巯醚(thioester）结构,从而实现标记。尽管胺基(如赖氨酸和精氨酸的侧链）也具有亲和性,但在中性或微酸性缓冲液中,马来酰亚胺对巯基的选择性标记显著(反应速度比胺基快超过1000倍）,标记反应迅速、选择性高且产率优良。此外,巯基是生物分子中广泛存在的官能团,例如蛋白质中的半胱氨酸(cysteine）和双硫键等。因此,马来酰亚胺/巯基(maleimide/thiol）标记已成为仅次于 NHS/胺基标记的最常用生物连接反应。
存储条件	keep away from direct sunlight \| store at -20°C \| Shipping with blue ice.
溶解度	DMSO : 40 mg/mL (53.95 mM), Sonication is recommended.