| 名称 | mCherry mRNA |
| 描述 | mCherry-mRNA is an mRNA molecule capped at the 5' end, polyadenylated at the 3' end, and modified with N1-Me-Pseudo UTP, mimicking the processed mature mRNA found in eukaryotes. The mRNA is capped co-transcriptionally using 3'-O-Me-GAG, forming a Cap1 structure which enhances mRNA stability and translation efficiency. The incorporation of the modified nucleotide N1-Me-Pseudo UTP reduces innate immune stimulation and increases mRNA stability. The addition of the poly(A) tail further stabilizes the mRNA and enhances its translation initiation efficiency. |
| 体外活性 | 每百万个细胞添加1ug CD5/LNP-mCherry,24小时后通过流式细胞术检测mCherry表达情况。静息态T细胞的mCherry表达水平较低(约12%）,但经αCD3/CD28磁珠激活后,其表达率显著提升至75-80%[1]。 |
| 体内活性 | 在Naive小鼠中静脉注射CD5/LNP-mCherry(10 ug）,24 小时后流式细胞术显示 脾脏和淋巴结中有5%至15%的CD4+和CD8+ T细胞表达mCherry[1]。 |
| 存储条件 | store at -80°C for 2 years |
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