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橙色荧光蛋白tdTomato mRNA
tdTomato mRNA
TMRH-0005-100μg
TargetMol
¥2280.00
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商品介绍

Product Introduction

Bioactivity

名称tdTomato mRNA
描述tdTomato-mRNA is an mRNA molecule capped at the 5' end, polyadenylated at the 3' end, and modified with N1-Me-Pseudo UTP, mimicking the processed mature mRNA found in eukaryotes. The mRNA is capped co-transcriptionally using 3'-O-Me-GAG, forming a Cap1 structure which enhances mRNA stability and translation efficiency. The incorporation of the modified nucleotide N1-Me-Pseudo UTP reduces innate immune stimulation and increases mRNA stability. The addition of the poly(A) tail further stabilizes the mRNA and enhances its translation initiation efficiency.
体外活性在HeLa cells 中转染tdTomato mRNA(100 ng)24小时后进行荧光检测,结果显示出明显的红色荧光信号[1]。
体内活性在野生型C57BL / 6小鼠中注射tdTomato mRNA LNP-1(0.3 mg/kg,静脉注射),24小时后并未检测到肝脏、脾脏和肺器官中的tdTomato荧光信号,这表明针对LNP-1,需要采用一种替代方法来测量整个器官中的mRNA翻译[1]。
存储条件store at -80°C for 2 years

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