| 名称 | tdTomato mRNA |
| 描述 | tdTomato-mRNA is an mRNA molecule capped at the 5' end, polyadenylated at the 3' end, and modified with N1-Me-Pseudo UTP, mimicking the processed mature mRNA found in eukaryotes. The mRNA is capped co-transcriptionally using 3'-O-Me-GAG, forming a Cap1 structure which enhances mRNA stability and translation efficiency. The incorporation of the modified nucleotide N1-Me-Pseudo UTP reduces innate immune stimulation and increases mRNA stability. The addition of the poly(A) tail further stabilizes the mRNA and enhances its translation initiation efficiency. |
| 体外活性 | 在HeLa cells 中转染tdTomato mRNA(100 ng)24小时后进行荧光检测,结果显示出明显的红色荧光信号[1]。 |
| 体内活性 | 在野生型C57BL / 6小鼠中注射tdTomato mRNA LNP-1(0.3 mg/kg,静脉注射),24小时后并未检测到肝脏、脾脏和肺器官中的tdTomato荧光信号,这表明针对LNP-1,需要采用一种替代方法来测量整个器官中的mRNA翻译[1]。 |
| 存储条件 | store at -80°C for 2 years |